Journal: Frontiers in Pharmacology

Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism

doi: 10.3389/fphar.2026.1706420

Figure Lengend Snippet: PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human normal mammary epithelial cells MCF10A (ATCC, USA) were cultured in DMEM/F12 (Procell) supplemented with HS (5%), EGF (20 ng/mL), hydrocortisone (0.5 μg/mL), insulin (0.5 μg/mL), NEAA (1%), and P/S (1%).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay

Journal: bioRxiv

Article Title: Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates

doi: 10.64898/2026.01.29.702607

Figure Lengend Snippet: (A) 3D morphogenesis assays of MCF10A cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).

Article Snippet: HeLa cells (cat. #CCL-2), MCF10A human mammary epithelial cells (cat. #CRL-10317), MDA-MB-231 human breast cancer epithelial cells (cat. #HTB-26) and 293T human kidney epithelial cells (cat. #CRL-3216) were from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Infection, Construct, Cell Culture, Staining, Marker, Incubation